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KMID : 0811720080120060323
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Korean Journal of Physiology & Pharmacology
2008 Volume.12 No. 6 p.323 ~ p.330
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Voltage-dependent Ca2＋ Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach
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Kim Young-Chul
Lee Sang-Jin
Park Seon-Mee
Youn Sei-Jin
Lee Sang-Jeon
Yun Hyo-Yung
Choi Woong
Xu Wen-Xie
Suzuki Hikaru
Hashitani Hikaru
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Abstract
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The properties of voltage dependent Ca2＋ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min?1, respectively, in the presence of 1?M nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under K＋-rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With Cs＋-rich pipette solution at Vh=?80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1?M) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca2＋ currents (VDCCL).
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KEYWORD
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Gastrointestinal (GI) tract, Stomach, Interstitial cells of Cajal (ICC), Voltage-dependent Ca2＋ current (VDCC)
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